Loss of microRNA?147 function alleviates synovial inflammation through ZNF148 in rheumatoid and experimental arthritis
نویسندگان
چکیده
MicroRNA-147 (miR-147) had been previously found induced in synoviocytes by inflammatory stimuli derived from T cells experimental arthritis. This study was designed to verify whether loss of its function might alleviate events joints and rheumatoid arthritis (RA). Dark Agouti (DA) rats were injected intradermally with pristane induce arthritis, rno-miR-147 antagomir locally administrated into individual ankle compared negative control or rno-miR-155-5p (potential positive control). Arthritis onset, macroscopic severity, pathological changes monitored. While vitro, gain hsa-miR-147b-3p/hsa-miR-155-5p ZNF148 achieved human synovial fibroblast cell line SW982 RA fibroblasts (RASF). The expression miRNAs mRNAs detected using RT-quantitative PCR, protein Western blotting. Anti-miR-147 therapy could the especially for synovitis joint destruction Gain TNF-? stimulated RASF upregulate, contrast, downregulate gene TNF-?, IL-6, MMP3, MMP13. Hence, such alteration participate inflammation destruction. RNAi ZNF148, a miR-147's target, increased MMP13 cells. Also, mRNA sequencing data showed that hsa-miR-147b-3p mimic siRNA commonly regulated CCL3 DEPTOR as well some inflammation-related pathways. Taken together, miR-147b-3p contributes through repressing
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ژورنال
عنوان ژورنال: European Journal of Immunology
سال: 2021
ISSN: ['1521-4141', '0014-2980']
DOI: https://doi.org/10.1002/eji.202048850